Objective. The goal of the present study was to investigate the effect of IL-12 expressed in plasmid on the Th1 cytokine profile in\nan experimental HPV16-positive murine tumor model and the association with the IL-12�s antitumor effect. Methods. Mice were\ninjected with BMK-16/myc cells to establish HPV16-positive tumor and then pNGVL3-mIL-12 plasmid; pcDNA3 plasmid or PBS\nwas injected directly into tumor site. The antitumor effect of the treatment was evaluated and the cytokines expression profile in\neach tumor tissue was analyzed. Results. Treatment with pNGVL3-mIL-12 plasmid had a significant antitumor effect, and a Th2-\nTh3-type cytokines prolife was detected in the murine tumor model with expression of the cytokines IL-10, IL-4, and TGF-??1.\nHowever, after the tumor was treated with three intratumoral injections of plasmid containing IL-12 cDNA, it showed a cytokine\nprofile associated withTh1 with expression of IL-2, IL-12, and IFN-?? cytokines and reduced expression of IL-10, IL-4, and TGF-??1.\nConclusions.The treatment with the IL-12 gene in the experimental HPV16-positive tumor model promoted the activation of the\ncellular immune response via expression of a Th1-type cytokine profile and was associated with the inhibition of tumor growth.\nThus, IL-12 treatment represents a novel approach for gene therapy against cervical cancer.
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